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Human AOC3/VAP-1 Recombinant Protein (C-His)
Human AOC3/VAP-1 Recombinant Protein (C-His)
Human AOC3/VAP-1 Recombinant Protein (C-His)
Human AOC3/VAP-1 Recombinant Protein (C-His)
Цена(USD):
Спецификация:
  • 50ug
  • 100ug
  • 1mg
Количество:
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  • Обзор

  • Изображения

  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерHS721011
Описание
Recombinant Human AOC3/VAP-1 Protein, C-His (HS721011) expressed in Insect Cells, spanning Gly27-Asn763. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессииInsect cells
Номер доступаQ16853
Длина белкаGly27-Asn763
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыHomo sapiens (Human)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса 84.75 kDa
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияMembrane primary amine oxidase, AOC3, SSAO, Semicarbazide-sensitive amine oxidase, Vascular adhesion protein 1, VAP-1, HPAO, Copper amine oxidase, VAP1
Фон

Amine oxidase [copper-containing] 3 (AOC3/VAP-1) is a ~84 kDa protein. Catalyzes the oxidative deamination of primary amines to the corresponding aldehydes with the concomitant production of hydrogen peroxide and ammonia. Has a preference for the primary monoamines methylamine and benzylamine. Could also act on 2-phenylethylamine but much less efficiently. At endothelial cells surface can also function as a cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion.

1. Kaitaniemi, S. et al. (2009) Cellular and molecular life sciences : CMLS 66, 2743-57. PMID: 19588076
2. Bligt-Lindén, E. et al. (2013) Journal of medicinal chemistry 56, 9837-48. PMID: 24304424
3. Smith, DJ. et al. (1998) The Journal of experimental medicine 188, 17-27. PMID: 9653080
4. Salmi, M. et al. (1997) The Journal of experimental medicine 186, 589-600. PMID: 9254657
Примечание For research use only.
Изображения
  • Human AOC3/VAP-1 Recombinant Protein (C-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Human AOC3/VAP-1 Protein, C-His

  • Human AOC3/VAP-1 Recombinant Protein (C-His)

    Bioactivity

    Detects Human VAP-1/AOC3 Antibody (TK8-14) in indirect ELISAs.

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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