Human APLNR/APJ Recombinant Protein (N-His) (HW667011)

Name: Human APLNR/APJ Recombinant Protein (N-His)
Brand: abinScience
SKU: HW667011
Price: 264 USD
Availability: InStock

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Aviso · Catálogo HW667011 ha sido descontinuado.

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Vista general

Número de catálogo	HW667011
Descripción	Recombinant Human APLNR/APJ Protein, N-His (HW667011) expressed in Insect Cells, spanning Met1-Asp380. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	Insect cells
Número de acceso	P35414
Longitud de proteína	Met1-Asp380
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	G-protein coupled receptor HG11, Apelin receptor, AGTRL1, APJ, Angiotensin receptor-like 1, APLNR, G-protein coupled receptor APJ
Fondo	Apelin receptor (APLNR/APJ) is a ~42 kDa protein. G protein-coupled receptor for peptide hormones apelin (APLN) and apelin receptor early endogenous ligand (APELA/ELA), that plays a role in the regulation of normal cardiovascular function and fluid homeostasis. When acting as apelin receptor, activates both G(i) protein pathway that inhibits adenylate cyclase activity, and the beta-arrestin pathway that promotes internalization of the receptor. APLNR/APJ also functions as mechanoreceptor that is activated by pathological stimuli in a G-protein-independent fashion to induce beta-arrestin signaling, hence eliciting cardiac hypertrophy. However, the presence of apelin ligand blunts cardiac hypertrophic induction from APLNR/APJ on response to pathological stimuli. Plays a key role in early development such as gastrulation, blood vessels formation and heart morphogenesis by acting as a APELA receptor. 1. Cayabyab, M. et al. (2000) Journal of virology 74, 11972-6. PMID: 11090199 2. Scimia, MC. et al. (2012) Nature 488, 394-8. PMID: 22810587 3. Wang, Z. et al. (2015) Scientific reports 5, 8170. PMID: 25639753 4. Yang, P. et al. (2017) Circulation 135, 1160-1173. PMID: 28137936 5. Yue, Y. et al. (2022) Nature structural & molecular biology 29, 688-697. PMID: 35817871 6. Wang, WW. et al. (2024) Cell 187, 1460-1475.e20. PMID: 38428423
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human APLNR/APJ Protein, N-His

Referencias

Datasheet

Human APLNR/APJ Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

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Volume

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g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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