Anti-APLNR/APJ Polyclonal Antibody (HW667014)

Name: Anti-APLNR/APJ Polyclonal Antibody
Brand: abinScience
SKU: HW667014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HW667014

Description

Anti-APLNR/APJ Polyclonal Antibody (HW667014) is a rabbit polyclonal antibody detecting APLNR. Suitable for Human.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant APLNR/APJ (Glu2-Ser26&Leu167-Gly200&Tyr309-Asp380).

Target

AGTRL1, APJ, APLNR, Angiotensin receptor-like 1, Apelin receptor, G-protein coupled receptor APJ, G-protein coupled receptor HG11

Endotoxin level

Please contact with the lab for this information.

Observed Molecular Weight

42 kDa

Purification

Purified by antigen affinity column.

Accession

P35414

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Apelin receptor (APLNR/APJ) is a ~42 kDa protein. G protein-coupled receptor for peptide hormones apelin (APLN) and apelin receptor early endogenous ligand (APELA/ELA), that plays a role in the regulation of normal cardiovascular function and fluid homeostasis. When acting as apelin receptor, activates both G(i) protein pathway that inhibits adenylate cyclase activity, and the beta-arrestin pathway that promotes internalization of the receptor. APLNR/APJ also functions as mechanoreceptor that is activated by pathological stimuli in a G-protein-independent fashion to induce beta-arrestin signaling, hence eliciting cardiac hypertrophy. However, the presence of apelin ligand blunts cardiac hypertrophic induction from APLNR/APJ on response to pathological stimuli. Plays a key role in early development such as gastrulation, blood vessels formation and heart morphogenesis by acting as a APELA receptor.

1. Cayabyab, M. et al. (2000) Journal of virology 74, 11972-6. PMID: 11090199

2. Scimia, MC. et al. (2012) Nature 488, 394-8. PMID: 22810587

3. Wang, Z. et al. (2015) Scientific reports 5, 8170. PMID: 25639753

4. Yang, P. et al. (2017) Circulation 135, 1160-1173. PMID: 28137936

5. Yue, Y. et al. (2022) Nature structural & molecular biology 29, 688-697. PMID: 35817871

6. Wang, WW. et al. (2024) Cell 187, 1460-1475.e20. PMID: 38428423

Note

For research use only

Images

References

Datasheet

Anti-APLNR/APJ Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-APLNR/APJ Polyclonal Antibody (HW667014)

Overview

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References

Datasheet

SDS

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