Human ARF6 Recombinant Protein (N-His) (HX239012)

Name: Human ARF6 Recombinant Protein (N-His)
Brand: abinScience
SKU: HX239012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	HX239012
Описание	Recombinant Human ARF6 Protein, N-His (HX239012) expressed in E. coli, spanning Met1-Ser175. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P62330
Длина белка	Met1-Ser175
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	22.25 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	ARF6, ADP-ribosylation factor 6
Фон	ADP-ribosylation factor 6 (ARF6) is a ~20 kDa protein. GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. GTP-bound form plays an important role in the transport of multiple palmitoylated proteins form the Golgi to the plasma membrane. Required for normal completion of mitotic cytokinesis. Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension. 1. Pasqualato, S. et al. (2001) EMBO reports 2, 234-8. PMID: 11266366 2. Fang, Z. et al. (2006) Molecular & cellular proteomics : MCP 5, 1437-49. PMID: 16737952 3. Ha, VL. et al. (2008) The Journal of biological chemistry 283, 14915-26. PMID: 18400762 4. Selyunin, AS. et al. (2011) Nature 469, 107-11. PMID: 21170023 5. Kosciuk, T. et al. (2020) Nature communications 11, 1067. PMID: 32103017 6. D'Souza-Schorey, C. et al. (1995) Experimental cell research 221, 153-9. PMID: 7589240 7. Wang, J. et al. (2023) Journal of cell science 136. PMID: 37461827 8. Gauthier-Campbell, C. et al. (2004) Molecular biology of the cell 15, 2205-17. PMID: 14978216 9. Chau, DD. et al. (2022) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36, e22594. PMID: 36250347 10. Chen, KJ. et al. (2022) Journal of cell science 135. PMID: 36017701
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human ARF6 Protein, N-His

Ссылки

Даташит

Human ARF6 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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