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Anti-Human ARF6 Polyclonal Antibody (HX239014)

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Overview
Catalog No.HX239014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ARF6 (Met1-Ser175).
Target ARF6, ADP-ribosylation factor 6
Purification Purified by antigen affinity column.
Accession P62330
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

ADP-ribosylation factor 6 (ARF6) is a ~20 kDa protein. GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. GTP-bound form plays an important role in the transport of multiple palmitoylated proteins form the Golgi to the plasma membrane. Required for normal completion of mitotic cytokinesis. Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension.

1. Pasqualato, S. et al. (2001) EMBO reports 2, 234-8. PMID: 11266366
2. Fang, Z. et al. (2006) Molecular & cellular proteomics : MCP 5, 1437-49. PMID: 16737952
3. Ha, VL. et al. (2008) The Journal of biological chemistry 283, 14915-26. PMID: 18400762
4. Selyunin, AS. et al. (2011) Nature 469, 107-11. PMID: 21170023
5. Kosciuk, T. et al. (2020) Nature communications 11, 1067. PMID: 32103017
6. D'Souza-Schorey, C. et al. (1995) Experimental cell research 221, 153-9. PMID: 7589240
7. Wang, J. et al. (2023) Journal of cell science 136. PMID: 37461827
8. Gauthier-Campbell, C. et al. (2004) Molecular biology of the cell 15, 2205-17. PMID: 14978216
9. Chau, DD. et al. (2022) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36, e22594. PMID: 36250347
10. Chen, KJ. et al. (2022) Journal of cell science 135. PMID: 36017701
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human ARF6 Polyclonal Antibody

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