Human ATG4D Recombinant Protein (N-His) (HC229012)

Name: Human ATG4D Recombinant Protein (N-His)
Brand: abinScience
SKU: HC229012
Price: 211 USD
Availability: InStock

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100ug
1mg

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Каталожный номер	HC229012
Описание	Recombinant Human ATG4D Protein, N-His (HC229012) expressed in E. coli, spanning Ser92-Ser420. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q86TL0
Длина белка	Ser92-Ser420
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	39.43 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	APG4D, ATG4D, AUT-like 4 cysteine endopeptidase, AUTL4, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, Cysteine protease ATG4D, Cysteine protease ATG4D, mitochondrial, EC:3.4.22.-, HsAPG4D
Фон	Cysteine protease ATG4D is a ~52 kDa protein. Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. In addition to the protease activity, also mediates delipidation of ATG8 family proteins. Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy. 1. Li, M. et al. (2011) The Journal of biological chemistry 286, 7327-38. PMID: 21177865 2. Kauffman, KJ. et al. (2018) Autophagy 14, 992-1010. PMID: 29458288 3. Agrotis, A. et al. (2019) Autophagy 15, 976-997. PMID: 30661429 4. Durgan, J. et al. (2021) Molecular cell 81, 2031-2040.e8. PMID: 33909989 5. Nguyen, TN. et al. (2021) Molecular cell 81, 2013-2030.e9. PMID: 33773106
Примечание	For research use only

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Human ATG4D Recombinant Protein (N-His).pdf

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