Anti-Human ATG4D Polyclonal Antibody (HC229014)

Name: Anti-Human ATG4D Polyclonal Antibody
Brand: abinScience
SKU: HC229014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HC229014

Description

Anti-Human ATG4D Polyclonal Antibody (HC229014) is a rabbit polyclonal antibody detecting ATG4D. Suitable for Human.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ATG4D (Ser92-Ser420).

Target

APG4D, ATG4D, AUT-like 4 cysteine endopeptidase, AUTL4, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, Cysteine protease ATG4D, Cysteine protease ATG4D, mitochondrial, EC:3.4.22.-, HsAPG4D

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q86TL0

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Cysteine protease ATG4D is a ~52 kDa protein. Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. In addition to the protease activity, also mediates delipidation of ATG8 family proteins. Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy.

1. Li, M. et al. (2011) The Journal of biological chemistry 286, 7327-38. PMID: 21177865

2. Kauffman, KJ. et al. (2018) Autophagy 14, 992-1010. PMID: 29458288

3. Agrotis, A. et al. (2019) Autophagy 15, 976-997. PMID: 30661429

4. Durgan, J. et al. (2021) Molecular cell 81, 2031-2040.e8. PMID: 33909989

5. Nguyen, TN. et al. (2021) Molecular cell 81, 2013-2030.e9. PMID: 33773106

Note

For research use only

Images

References

Datasheet

Anti-Human ATG4D Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human ATG4D Polyclonal Antibody (HC229014)

Overview

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References

Datasheet

SDS

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