Human ATP2C2 Recombinant Protein (N-GST) (HF519012)

Name: Human ATP2C2 Recombinant Protein (N-GST)
Brand: abinScience
SKU: HF519012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HF519012
Description	Recombinant Human ATP2C2 Protein, N-GST (HF519012) expressed in E. coli, spanning Met1-Tyr106. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	O75185
Longueur de protéine	Met1-Tyr106
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	38.89 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	ATPase 2C2, Secretory pathway Ca(2+)-ATPase 2, ATP2C2, KIAA0703, Calcium-transporting ATPase type 2C member 2, SPCA2
Contexte	Calcium-transporting ATPase type 2C member 2 (ATP2C2) is a ~103 kDa protein. ATP-driven pump that supplies the Golgi apparatus with Ca(2+) and Mn(2+) ions, both essential cofactors for processing and trafficking of newly synthesized proteins in the secretory pathway. Within a catalytic cycle, acquires Ca(2+) or Mn(2+) ions on the cytoplasmic side of the membrane and delivers them to the lumenal side. The transfer of ions across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state. Induces Ca(2+) influx independently of its ATP-driven pump function. At the basolateral membrane of mammary epithelial cells, interacts with Ca(2+) channel ORAI1 and mediates Ca(2+) entry independently of the Ca(2+) content of endoplasmic reticulum or Golgi stores. 1. Xiang, M. et al. (2005) The Journal of biological chemistry 280, 11608-14. PMID: 15677451 2. Vanoevelen, J. et al. (2005) The Journal of biological chemistry 280, 22800-8. PMID: 15831496 3. Dode, L. et al. (2006) The Journal of biological chemistry 281, 3182-9. PMID: 16332677 4. Chen, J. et al. (2019) The Journal of biological chemistry 294, 7878-7891. PMID: 30923126 5. Feng, M. et al. (2010) Cell 143, 84-98. PMID: 20887894 6. Cross, BM. et al. (2013) PloS one 8, e67348. PMID: 23840669
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human ATP2C2 Protein, N-GST

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Human ATP2C2 Recombinant Protein (N-GST).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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