Human ATP2C2 Recombinant Protein (N-His) (HF519022)

Name: Human ATP2C2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HF519022
Price: 211 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HF519022
Description	Recombinant Human ATP2C2 Protein, N-His (HF519022) expressed in E. coli, spanning Glu388-Asp576. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O75185
Protein length	Glu388-Asp576
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	22.92 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ATP2C2, ATPase 2C2, Ca(2+)/Mn(2+)-ATPase 2C2, Calcium-transporting ATPase type 2C member 2, EC:7.2.2.10, KIAA0703, SPCA2, Secretory pathway Ca(2+)-transporting ATPase type 2
Background	Calcium-transporting ATPase type 2C member 2 is a ~103 kDa protein. ATP-driven pump that supplies the Golgi apparatus with Ca(2+) and Mn(2+) ions, both essential cofactors for processing and trafficking of newly synthesized proteins in the secretory pathway. Within a catalytic cycle, acquires Ca(2+) or Mn(2+) ions on the cytoplasmic side of the membrane and delivers them to the lumenal side. The transfer of ions across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state. Induces Ca(2+) influx independently of its ATP-driven pump function. At the basolateral membrane of mammary epithelial cells, interacts with Ca(2+) channel ORAI1 and mediates Ca(2+) entry independently of the Ca(2+) content of endoplasmic reticulum or Golgi stores. 1. Xiang, M. et al. (2005) The Journal of biological chemistry 280, 11608-14. PMID: 15677451 2. Vanoevelen, J. et al. (2005) The Journal of biological chemistry 280, 22800-8. PMID: 15831496 3. Dode, L. et al. (2006) The Journal of biological chemistry 281, 3182-9. PMID: 16332677 4. Chen, J. et al. (2019) The Journal of biological chemistry 294, 7878-7891. PMID: 30923126 5. Feng, M. et al. (2010) Cell 143, 84-98. PMID: 20887894 6. Cross, BM. et al. (2013) PloS one 8, e67348. PMID: 23840669
Note	For research use only

Images

References

Datasheet

Human ATP2C2 Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Human ATP2C2 Recombinant Protein (N-His) (HF519022)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us