Human BAG3 Recombinant Protein (N-GST) (HF640012)

Name: Human BAG3 Recombinant Protein (N-GST)
Brand: abinScience
SKU: HF640012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HF640012
Description	Recombinant Human BAG3 Protein, N-GST (HF640012) expressed in E. coli, spanning Gly421-Ala498. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	O95817
Longueur de protéine	Gly421-Ala498
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	35.71 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Docking protein CAIR-1, BAG3, Bcl-2-associated athanogene 3, BAG family molecular chaperone regulator 3, Bcl-2-binding protein Bis, BAG-3, BIS
Contexte	BAG family molecular chaperone regulator 3 (BAG3) is a ~61 kDa protein. Co-chaperone and adapter protein that connects different classes of molecular chaperones including heat shock proteins 70 (HSP70s), e.g. HSPA1A/HSP70 or HSPA8/HSC70, and small heat shock proteins (sHSPs), e.g. HSPB8. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from HSP70s, thereby triggering client protein release. Nucleotide release is mediated via BAG3 binding to the nucleotide-binding domain (NBD) of HSP70s, whereas client release is mediated via binding to the substrate-binding domain (SBD). 1. Rauch, JN. et al. (2017) Journal of molecular biology 429, 128-141. PMID: 27884606 2. Meister-Broekema, M. et al. (2018) Nature communications 9, 5342. PMID: 30559338 3. Rauch, JN. et al. (2016) The Journal of biological chemistry 291, 19848-57. PMID: 27474739 4. Takayama, S. et al. (1999) The Journal of biological chemistry 274, 781-6. PMID: 9873016 5. Lee, JH. et al. (1999) Oncogene 18, 6183-90. PMID: 10597216 6. Jin, YH. et al. (2015) Biochemical and biophysical research communications 464, 561-7. PMID: 26159920
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human BAG3 Protein, N-GST

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Human BAG3 Recombinant Protein (N-GST).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

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