Human BCR Recombinant Protein (N-His) (HY023012)

Name: Human BCR Recombinant Protein (N-His)
Brand: abinScience
SKU: HY023012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HY023012
Description	Recombinant Human BCR Protein, N-His (HY023012) expressed in E. coli, spanning Lys174-Asp331. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P11274
Longueur de protéine	Lys174-Asp331
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	19.91 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Renal carcinoma antigen NY-REN-26, BCR1, BCR, D22S11, Breakpoint cluster region protein
Contexte	Breakpoint cluster region protein (BCR) is a ~142 kDa protein. Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein (GAP) domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form. The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form. 1. Cho, YJ. et al. (2007) Molecular and cellular biology 27, 899-911. PMID: 17116687 2. Diekmann, D. et al. (1991) Nature 351, 400-2. PMID: 1903516 3. Chuang, TH. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 10282-6. PMID: 7479768 4. Dubash, AD. et al. (2013) The Journal of cell biology 202, 653-66. PMID: 23940119 5. Maru, Y. et al. (1991) Cell 67, 459-68. PMID: 1657398
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human BCR Protein, N-His

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Human BCR Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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