中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HC377022 |
|---|---|
| 説明 |
Recombinant Human BECN1 Protein, N-His (HC377022) expressed in E. coli, spanning Thr141-Lys450. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q14457 |
| タンパク質長 | Thr141-Lys450 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 38.79 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, GT197 |
| 背景 | Beclin-1 (BECN1) is a ~51 kDa protein. Plays a central role in autophagy. Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis. 1. Wei, Y. et al. (2008) Molecular cell 30, 678-88. PMID: 18570871 2. Strappazzon, F. et al. (2011) The EMBO journal 30, 1195-208. PMID: 21358617 3. Margariti, A. et al. (2013) The Journal of biological chemistry 288, 859-72. PMID: 23184933 4. Xia, P. et al. (2013) The EMBO journal 32, 2685-96. PMID: 23974797 5. Xu, C. et al. (2014) Autophagy 10, 2239-50. PMID: 25484083 6. Ashkenazi, A. et al. (2017) Nature 545, 108-111. PMID: 28445460 7. Wyatt, S. et al. (2023) Biochemistry 62, 2934-2951. PMID: 37776275 8. Sagona, AP. et al. (2010) Nature cell biology 12, 362-71. PMID: 20208530 9. Thoresen, SB. et al. (2010) Experimental cell research 316, 3368-78. PMID: 20643123 10. Liu, K. et al. (2016) The Journal of cell biology 212, 181-98. PMID: 26783301 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human BECN1 Protein, N-His
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
