Anti-BECN1 Polyclonal Antibody (HC377024)

Name: Anti-BECN1 Polyclonal Antibody
Brand: abinScience
SKU: HC377024
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC377024

Description

Anti-BECN1 Polyclonal Antibody (HC377024) is a rabbit polyclonal antibody detecting BECN1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human BECN1 (Thr141-Lys450).

Target

BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, GT197

Purification

Purified by antigen affinity column.

Accession

Q14457

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Beclin-1 (BECN1) is a ~51 kDa protein. Plays a central role in autophagy. Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis.

1. Wei, Y. et al. (2008) Molecular cell 30, 678-88. PMID: 18570871

2. Strappazzon, F. et al. (2011) The EMBO journal 30, 1195-208. PMID: 21358617

3. Margariti, A. et al. (2013) The Journal of biological chemistry 288, 859-72. PMID: 23184933

4. Xia, P. et al. (2013) The EMBO journal 32, 2685-96. PMID: 23974797

5. Xu, C. et al. (2014) Autophagy 10, 2239-50. PMID: 25484083

6. Ashkenazi, A. et al. (2017) Nature 545, 108-111. PMID: 28445460

7. Wyatt, S. et al. (2023) Biochemistry 62, 2934-2951. PMID: 37776275

8. Sagona, AP. et al. (2010) Nature cell biology 12, 362-71. PMID: 20208530

9. Thoresen, SB. et al. (2010) Experimental cell research 316, 3368-78. PMID: 20643123

10. Liu, K. et al. (2016) The Journal of cell biology 212, 181-98. PMID: 26783301

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Beclin-1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Mouse testis lysate
Lane 3: Mouse brain lysate
Lane 4: Mouse lung lysate
Lane 5: Rat testis lysate
Lane 6: Rat brain lysate
Lane 7: Rat lung lysate