ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Homo sapiens (Human)
Характер
Recombinant
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса
17.93 kDa
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия
bHLHe41, hDEC2, DEC2, bHLHb3, Enhancer-of-split and hairy-related protein 1, Class E basic helix-loop-helix protein 41, Class B basic helix-loop-helix protein 3, BHLHB3, SHARP1, SHARP-1, Differentially expressed in chondrocytes protein 2, BHLHE41
Фон
Class E basic helix-loop-helix protein 41 (BHLHE41) is a ~50 kDa protein. Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2.
1. Garriga-Canut, M. et al. (2001) The Journal of biological chemistry 276, 14821-8. PMID: 11278948
2. Kawamoto, T. et al. (2004) Biochemical and biophysical research communications 313, 117-24. PMID: 14672706
3. Li, Y. et al. (2004) The Biochemical journal 382, 895-904. PMID: 15193144
4. Sato, F. et al. (2004) European journal of biochemistry 271, 4409-19. PMID: 15560782
5. Nakashima, A. et al. (2008) Molecular and cellular biology 28, 4080-92. PMID: 18411297
6. Cho, Y. et al. (2009) Molecular pharmacology 76, 1360-9. PMID: 19786558
7. Pellegrino, R. et al. (2014) Sleep 37, 1327-36. PMID: 25083013
Примечание
For research use only.
Изображения
SDS-PAGE
SDS-PAGE for Recombinant Human BHLHE41 Protein, N-His
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