Anti-Human BHLHE41 Polyclonal Antibody (HP018014)

Name: Anti-Human BHLHE41 Polyclonal Antibody
Brand: abinScience
SKU: HP018014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HP018014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human BHLHE41 (Asp40-Leu174).

Target

bHLHe41, hDEC2, DEC2, bHLHb3, Enhancer-of-split and hairy-related protein 1, Class E basic helix-loop-helix protein 41, Class B basic helix-loop-helix protein 3, BHLHB3, SHARP1, SHARP-1, Differentially expressed in chondrocytes protein 2, BHLHE41

Purification

Purified by antigen affinity column.

Accession

Q9C0J9

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Class E basic helix-loop-helix protein 41 (BHLHE41) is a ~50 kDa protein. Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2.

1. Garriga-Canut, M. et al. (2001) The Journal of biological chemistry 276, 14821-8. PMID: 11278948

2. Kawamoto, T. et al. (2004) Biochemical and biophysical research communications 313, 117-24. PMID: 14672706

3. Li, Y. et al. (2004) The Biochemical journal 382, 895-904. PMID: 15193144

4. Sato, F. et al. (2004) European journal of biochemistry 271, 4409-19. PMID: 15560782

5. Nakashima, A. et al. (2008) Molecular and cellular biology 28, 4080-92. PMID: 18411297

6. Cho, Y. et al. (2009) Molecular pharmacology 76, 1360-9. PMID: 19786558

7. Pellegrino, R. et al. (2014) Sleep 37, 1327-36. PMID: 25083013

Note

For research use only.

Images

References

Datasheet

Anti-Human BHLHE41 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-Human BHLHE41 Polyclonal Antibody (HP018014)

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Datasheet

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