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| カタログ番号 | HT337012 |
|---|---|
| 説明 |
Recombinant Human BIRC5 Protein, N-His (HT337012) expressed in E. coli, spanning Met1-Asp142. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O15392 |
| タンパク質長 | Met1-Asp142 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 18.55 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | BIRC5, IAP4, Apoptosis inhibitor survivin, Apoptosis inhibitor 4, API4, Baculoviral IAP repeat-containing protein 5 |
| 背景 | Baculoviral IAP repeat-containing protein 5 (BIRC5) is a ~16 kDa protein. Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. 1. Habtemichael, N. et al. (2010) Molecular and cellular neurosciences 45, 297-305. PMID: 20627126 2. Knauer, SK. et al. (2010) Cell death & disease 1, e51. PMID: 21364656 3. Liu, Y. et al. (2015) The Journal of biological chemistry 290, 11843-52. PMID: 25778398 4. Kamran, M. et al. (2017) Oncogenesis 6, e298. PMID: 28218735 5. Li, F. et al. (1998) Nature 396, 580-4. PMID: 9859993 6. Vong, QP. et al. (2005) Science (New York, N.Y.) 310, 1499-504. PMID: 16322459 7. Wang, H. et al. (2010) The Journal of biological chemistry 285, 36129-37. PMID: 20826784 8. Yamagishi, Y. et al. (2010) Science (New York, N.Y.) 330, 239-43. PMID: 20929775 9. Xia, F. et al. (2008) Molecular and cellular biology 28, 5299-311. PMID: 18591255 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human BIRC5 Protein, N-His
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