Anti-BIRC5 Polyclonal Antibody (HT337014)

Name: Anti-BIRC5 Polyclonal Antibody
Brand: abinScience
SKU: HT337014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HT337014

Description

Anti-BIRC5 Polyclonal Antibody (HT337014) is a rabbit polyclonal antibody detecting BIRC5 in ELISA, IHC, WB. Suitable for Human, Dog, Cat, Bovine, and Cavia porcellus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human BIRC5 (Met1-Asp142).

Target

BIRC5, IAP4, Apoptosis inhibitor survivin, Apoptosis inhibitor 4, API4, Baculoviral IAP repeat-containing protein 5

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O15392

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Baculoviral IAP repeat-containing protein 5 (BIRC5) is a ~16 kDa protein. Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.

1. Habtemichael, N. et al. (2010) Molecular and cellular neurosciences 45, 297-305. PMID: 20627126

2. Knauer, SK. et al. (2010) Cell death & disease 1, e51. PMID: 21364656

3. Liu, Y. et al. (2015) The Journal of biological chemistry 290, 11843-52. PMID: 25778398

4. Kamran, M. et al. (2017) Oncogenesis 6, e298. PMID: 28218735

5. Li, F. et al. (1998) Nature 396, 580-4. PMID: 9859993

6. Vong, QP. et al. (2005) Science (New York, N.Y.) 310, 1499-504. PMID: 16322459

7. Wang, H. et al. (2010) The Journal of biological chemistry 285, 36129-37. PMID: 20826784

8. Yamagishi, Y. et al. (2010) Science (New York, N.Y.) 330, 239-43. PMID: 20929775

9. Xia, F. et al. (2008) Molecular and cellular biology 28, 5299-311. PMID: 18591255

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-BIRC5 polyclonal antibody at 1ug/mL on recombinant BIRC5 (Catalog No. HT337012).

References

Datasheet

Anti-BIRC5 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-BIRC5 Polyclonal Antibody (HT337014)

Overview

Images

References

Datasheet

SDS

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