ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies
Homo sapiens (Human)
Naturaleza
Recombinant
Nivel de endotoxinas
Please contact with the lab for this information.
Pureza
>90% as determined by SDS-PAGE.
Peso molecular predicho
24.28 kDa
Forma
Lyophilized
Buffer de almacenamiento
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos
FR, Green heme-binding protein, BLVRB, Flavin reductase (NADPH), FLR, NADPH-flavin reductase, GHBP, Biliverdin-IX beta-reductase, NADPH-dependent diaphorase, BVR-B, Biliverdin reductase B
Fondo
Flavin reductase (NADPH) (BLVRB) is a ~22 kDa protein. Enzyme that can both act as a NAD(P)H-dependent reductase and a S-nitroso-CoA-dependent nitrosyltransferase. Promotes fetal heme degradation during development. Also expressed in adult tissues, where it acts as a regulator of hematopoiesis, intermediary metabolism (glutaminolysis, glycolysis, TCA cycle and pentose phosphate pathway) and insulin signaling. Has a broad specificity oxidoreductase activity by catalyzing the NAD(P)H-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to fetal heme catabolism by catalyzing reduction of biliverdin IXbeta into bilirubin IXbeta in the liver.
1. Cunningham, O. et al. (2000) The Biochemical journal 345 Pt 2, 393-9. PMID: 10620517
2. Smith, LJ. et al. (2008) The Biochemical journal 411, 475-84. PMID: 18241201
3. Wu, S. et al. (2016) Blood 128, 699-709. PMID: 27207795
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