Human BMP7 Recombinant Protein (N-His) (HB664012)

Name: Human BMP7 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB664012
Price: 211 USD
Availability: InStock

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Numéro de catalogue	HB664012
Description	Recombinant Human BMP7 Protein, N-His (HB664012) expressed in E. coli, spanning Asp30-His431. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P18075
Longueur de protéine	Asp30-His431
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	48.42 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Bone morphogenetic protein 7, Eptotermin alfa, OP-1, OP1, BMP-7, Osteogenic protein 1, BMP7
Contexte	Bone morphogenetic protein 7 (BMP7) is a ~49 kDa protein. Growth factor of the TGF-beta superfamily that plays important role in various biological processes, including embryogenesis, hematopoiesis, neurogenesis and skeletal morphogenesis. Initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2A. Once all three components are bound together in a complex at the cell surface, ACVR2A phosphorylates and activates ACVR1. In turn, ACVR1 propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. For specific functions such as growth cone collapse in developing spinal neurons and chemotaxis of monocytes, also uses BMPR2 as type II receptor. 1. Perron, JC. et al. (2019) Biology open 8. PMID: 31208997 2. Greenwald, J. et al. (2003) Molecular cell 11, 605-17. PMID: 12667445 3. Macías-Silva, M. et al. (1998) The Journal of biological chemistry 273, 25628-36. PMID: 9748228 4. Groppe, J. et al. (2002) Nature 420, 636-42. PMID: 12478285 5. Brunmeir, R. et al. (2016) PLoS genetics 12, e1006474. PMID: 27923061 6. Arezes, J. et al. (2018) Blood 132, 1473-1477. PMID: 30097509
Note	For research use only.

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SDS-PAGE for Recombinant Human BMP7 Protein, N-His

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Human BMP7 Recombinant Protein (N-His).pdf

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