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| カタログ番号 | HS898022 |
|---|---|
| 説明 |
Recombinant Human BRD3 Protein, N-His (HS898022) expressed in E. coli, spanning Lys35-Pro141. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q15059 |
| タンパク質長 | Lys35-Pro141 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 15.11 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | BRD3, Bromodomain-containing protein 3, KIAA0043, RING3-like protein, RING3L |
| 背景 | Bromodomain-containing protein 3 is a ~79 kDa protein. Chromatin reader that recognizes and binds acetylated histones, thereby controlling gene expression and remodeling chromatin structures. Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation. In vitro, binds acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4. Involved in endoderm differentiation via its association with long non-coding RNA (lncRNA) DIGIT: BRD3 undergoes liquid-liquid phase separation upon binding to lncRNA DIGIT, promoting binding to histone H3 acetylated at 'Lys-18' (H3K18ac) to induce endoderm gene expression. Also binds non-histones acetylated proteins, such as GATA1 and GATA2: regulates transcription by promoting the binding of the transcription factor GATA1 to its targets. 1. LeRoy, G. et al. (2008) Molecular cell 30, 51-60. PMID: 18406326 2. Filippakopoulos, P. et al. (2012) Cell 149, 214-31. PMID: 22464331 3. Li, Y. et al. (2016) Molecular cell 62, 181-193. PMID: 27105114 4. Daneshvar, K. et al. (2020) Nature cell biology 22, 1211-1222. PMID: 32895492 5. Wai, DCC. et al. (2018) The Journal of biological chemistry 293, 7160-7175. PMID: 29567837 |
| 注意事項 | For research use only |
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