Human BRD3 Recombinant Protein (N-His-SUMO) (HS898012)

Name: Human BRD3 Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: HS898012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HS898012
Description	Recombinant Human BRD3 Protein, N-His-SUMO (HS898012) expressed in E. coli, spanning Lys35-Gln139. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q15059
Protein length	Lys35-Gln139
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	24.93 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	BRD3, RING3-like protein, Bromodomain-containing protein 3, KIAA0043, RING3L
Background	Bromodomain-containing protein 3 (BRD3) is a ~79 kDa protein. Chromatin reader that recognizes and binds acetylated histones, thereby controlling gene expression and remodeling chromatin structures. Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation. In vitro, binds acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4. Involved in endoderm differentiation via its association with long non-coding RNA (lncRNA) DIGIT: BRD3 undergoes liquid-liquid phase separation upon binding to lncRNA DIGIT, promoting binding to histone H3 acetylated at 'Lys-18' (H3K18ac) to induce endoderm gene expression. Also binds non-histones acetylated proteins, such as GATA1 and GATA2: regulates transcription by promoting the binding of the transcription factor GATA1 to its targets. 1. LeRoy, G. et al. (2008) Molecular cell 30, 51-60. PMID: 18406326 2. Filippakopoulos, P. et al. (2012) Cell 149, 214-31. PMID: 22464331 3. Li, Y. et al. (2016) Molecular cell 62, 181-193. PMID: 27105114 4. Daneshvar, K. et al. (2020) Nature cell biology 22, 1211-1222. PMID: 32895492 5. Wai, DCC. et al. (2018) The Journal of biological chemistry 293, 7160-7175. PMID: 29567837
Note	For research use only.

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SDS-PAGE for Recombinant Human BRD3 Protein, N-His-SUMO

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HS898012-SDS-PAGE-1.jpg

Datasheet

Human BRD3 Recombinant Protein (N-His-SUMO).pdf

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Human BRD3 Recombinant Protein (N-His-SUMO) (HS898012)

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