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| カタログ番号 | HF917012 |
|---|---|
| 説明 |
Recombinant Human C1R Protein, N-His (HF917012) expressed in E. coli, spanning Ala356-Asp705. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P00736 |
| タンパク質長 | Ala356-Asp705 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 41.89 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | C1R, Complement component 1 subcomponent r, Complement C1r subcomponent |
| 背景 | Complement C1r subcomponent is a ~80 kDa protein. Serine protease component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C1R catalyzes the first enzymatic step in the classical complement pathway: it is activated by the C1Q subcomplex of the C1 complex, which associates with IgG or IgM immunoglobulins complexed with antigens to form antigen-antibody complexes on the surface of pathogens. Immunoglobulin-binding promotes the autocatalytic cleavage and activation of C1R. Activated C1R then cleaves and activates C1S, the second protease of the classical complement pathway. It is unclear if C1R activates C1S within single, strained C1 complexes or between neighboring C1 complexes on surfaces. 1. Kardos, J. et al. (2008) Molecular immunology 45, 1752-60. PMID: 17996945 2. Bally, I. et al. (2009) The Journal of biological chemistry 284, 19340-8. PMID: 19473974 3. Ugurlar, D. et al. (2018) Science (New York, N.Y.) 359, 794-797. PMID: 29449492 4. Zwarthoff, SA. et al. (2021) Proceedings of the National Academy of Sciences of the United States of America 118. PMID: 34155115 5. Lacroix, M. et al. (2001) The Journal of biological chemistry 276, 36233-40. PMID: 11445589 6. Kardos, J. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 5202-8. PMID: 11673533 7. Major, B. et al. (2010) The Journal of biological chemistry 285, 11863-9. PMID: 20178990 8. Arlaud, GJ. et al. (1980) Biochimica et biophysica acta 616, 116-29. PMID: 6254570 9. McRae, BJ. et al. (1981) The Journal of biological chemistry 256, 12362-6. PMID: 6271784 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human C1R Protein, N-His
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