Anti-C1R Polyclonal Antibody (HF917014)

Name: Anti-C1R Polyclonal Antibody
Brand: abinScience
SKU: HF917014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HF917014

Description

Anti-C1R Polyclonal Antibody (HF917014) is a rabbit polyclonal antibody detecting C1R in ELISA, IHC, WB. Suitable for Human, Macaca fascicularis, Pan troglodytes, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human C1R (Ala356-Asp705).

Target

C1R, Complement component 1 subcomponent r, Complement C1r subcomponent

Purification

Purified by antigen affinity column.

Accession

P00736

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Complement C1r subcomponent is a ~80 kDa protein. Serine protease component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C1R catalyzes the first enzymatic step in the classical complement pathway: it is activated by the C1Q subcomplex of the C1 complex, which associates with IgG or IgM immunoglobulins complexed with antigens to form antigen-antibody complexes on the surface of pathogens. Immunoglobulin-binding promotes the autocatalytic cleavage and activation of C1R. Activated C1R then cleaves and activates C1S, the second protease of the classical complement pathway. It is unclear if C1R activates C1S within single, strained C1 complexes or between neighboring C1 complexes on surfaces.

1. Kardos, J. et al. (2008) Molecular immunology 45, 1752-60. PMID: 17996945

2. Bally, I. et al. (2009) The Journal of biological chemistry 284, 19340-8. PMID: 19473974

3. Ugurlar, D. et al. (2018) Science (New York, N.Y.) 359, 794-797. PMID: 29449492

4. Zwarthoff, SA. et al. (2021) Proceedings of the National Academy of Sciences of the United States of America 118. PMID: 34155115

5. Lacroix, M. et al. (2001) The Journal of biological chemistry 276, 36233-40. PMID: 11445589

6. Kardos, J. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 5202-8. PMID: 11673533

7. Major, B. et al. (2010) The Journal of biological chemistry 285, 11863-9. PMID: 20178990

8. Arlaud, GJ. et al. (1980) Biochimica et biophysica acta 616, 116-29. PMID: 6254570

9. McRae, BJ. et al. (1981) The Journal of biological chemistry 256, 12362-6. PMID: 6271784

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-C1R polyclonal antibody at 1ug/mL on recombinant C1R (Catalog No. HF917012).

References

Datasheet

Anti-C1R Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-C1R Polyclonal Antibody (HF917014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us