Human CCND1 Recombinant Protein (N-His) (HB241012)

Name: Human CCND1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB241012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HB241012
Description	Recombinant Human CCND1 Protein, N-His (HB241012) expressed in E. coli, spanning Glu66-Arg245. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P24385
Longueur de protéine	Glu66-Arg245
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	22.98 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	B-cell lymphoma 1 protein, G1/S-specific cyclin-D1, BCL1, PRAD1, CCND1, PRAD1 oncogene, BCL-1 oncogene, BCL-1
Contexte	G1/S-specific cyclin-D1 (CCND1) is a ~33 kDa protein. Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also a substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. 1. Xiong, Y. et al. (1991) Cell 65, 691-9. PMID: 1827756 2. Lew, DJ. et al. (1991) Cell 66, 1197-206. PMID: 1833066 3. Santra, MK. et al. (2009) Nature 459, 722-5. PMID: 19412162 4. Simoneschi, D. et al. (2021) Nature 592, 789-793. PMID: 33854235 5. Meyerson, M. et al. (1994) Molecular and cellular biology 14, 2077-86. PMID: 8114739 6. Bates, S. et al. (1994) Oncogene 9, 71-9. PMID: 8302605 8. LaBaer, J. et al. (1997) Genes & development 11, 847-62. PMID: 9106657
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human CCND1 Protein, N-His

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Human CCND1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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