Anti-Human Cyclin D1 Polyclonal Antibody (HB241024)

Name: Anti-Human Cyclin D1 Polyclonal Antibody
Brand: abinScience
SKU: HB241024
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB241024

Description

Anti-Human Cyclin D1 Polyclonal Antibody (HB241024) is a rabbit polyclonal antibody detecting Cyclin D1 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, Human, Gallus gallus, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human Cyclin D1 (Met1-Ile295).

Target

Cyclin D1

Purification

Purified by antigen affinity column.

Accession

P24385

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1

Background

G1/S-specific cyclin-D1 (Cyclin D1) is a ~33 kDa protein. Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also a substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity.

1. Xiong, Y. et al. (1991) Cell 65, 691-9. PMID: 1827756

2. Lew, DJ. et al. (1991) Cell 66, 1197-206. PMID: 1833066

3. Santra, MK. et al. (2009) Nature 459, 722-5. PMID: 19412162

4. Simoneschi, D. et al. (2021) Nature 592, 789-793. PMID: 33854235

5. Meyerson, M. et al. (1994) Molecular and cellular biology 14, 2077-86. PMID: 8114739

6. Bates, S. et al. (1994) Oncogene 9, 71-9. PMID: 8302605

8. LaBaer, J. et al. (1997) Genes & development 11, 847-62. PMID: 9106657

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Cyclin D1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HCT116 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: MCF-7 cell lysate
Lane 6: Lncap cell lysate