中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HB241012 |
|---|---|
| 説明 |
Recombinant Human CCND1 Protein, N-His (HB241012) expressed in E. coli, spanning Glu66-Arg245. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P24385 |
| タンパク質長 | Glu66-Arg245 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 22.98 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | B-cell lymphoma 1 protein, G1/S-specific cyclin-D1, BCL1, PRAD1, CCND1, PRAD1 oncogene, BCL-1 oncogene, BCL-1 |
| 背景 | G1/S-specific cyclin-D1 (CCND1) is a ~33 kDa protein. Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also a substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. 1. Xiong, Y. et al. (1991) Cell 65, 691-9. PMID: 1827756 2. Lew, DJ. et al. (1991) Cell 66, 1197-206. PMID: 1833066 3. Santra, MK. et al. (2009) Nature 459, 722-5. PMID: 19412162 4. Simoneschi, D. et al. (2021) Nature 592, 789-793. PMID: 33854235 5. Meyerson, M. et al. (1994) Molecular and cellular biology 14, 2077-86. PMID: 8114739 6. Bates, S. et al. (1994) Oncogene 9, 71-9. PMID: 8302605 8. LaBaer, J. et al. (1997) Genes & development 11, 847-62. PMID: 9106657 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CCND1 Protein, N-His
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
