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Human CD111/NECTIN1 Recombinant Protein (C-His) (HS747011)

Human CD111/NECTIN1 Recombinant Protein (C-His)
Human CD111/NECTIN1 Recombinant Protein (C-His)
Human CD111/NECTIN1 Recombinant Protein (C-His)
Human CD111/NECTIN1 Recombinant Protein (C-His)
Precio(USD):
Especificación:
  • 50ug
  • 100ug
  • 1mg
Número:
Contáctenos
  • Vista general

  • Imágenes

  • Referencias

  • Datasheet

  • SDS

Vista general
Número de catálogoHS747011
Descripción
Recombinant Human CD111/NECTIN1 Protein, C-His (HS747011) expressed in Mammalian Cells, spanning Met1-Thr334.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresiónMammalian cells
Número de accesoQ15223
Longitud de proteínaMet1-Thr334
AplicacionesELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspeciesHomo sapiens (Human)
Naturaleza Recombinant
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Peso molecular predicho 38.02 kDa
Forma Lyophilized
Buffer de almacenamiento Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosHerpesvirus entry mediator C, CD111, Herpes virus entry mediator C, HIgR, Poliovirus receptor-related protein 1, PRR1, NECTIN1, Nectin cell adhesion molecule 1, Nectin-1, HveC, HVEC, Herpesvirus Ig-like receptor, PVRL1
Fondo

Nectin-1 (CD111/NECTIN1) is a ~57 kDa protein. Cell adhesion molecule that promotes cell-cell contacts and plays important roles in the development of the nervous system. Acts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between NECTIN1 and NECTIN3 and between NECTIN1 and NECTIN4. Involved in axon guidance by promoting contacts between the commissural axons and the floor plate cells. Involved in synaptogegesis.

1. Narita, H. et al. (2011) The Journal of biological chemistry 286, 12659-69. PMID: 21325282
Nota For research use only.
Imágenes
  • Human CD111/NECTIN1 Recombinant Protein (C-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Human CD111/NECTIN1 Protein, C-His

  • Human CD111/NECTIN1 Recombinant Protein (C-His)

    Bioactivity

    Immobilized HV985021 Human NECTIN4/PVRL4 Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HS747011 Human CD111/NECTIN1 Recombinant Protein (C-His), the EC 50 is 13.4 μg/mL.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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