Recombinant Human CD119/IFNGR1 Protein, C-His (HB990011) expressed in Mammalian Cells, spanning Met1-Gly245.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии
Mammalian cells
Номер доступа
P15260
Длина белка
Met1-Gly245
Применения
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Homo sapiens (Human)
Характер
Recombinant
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>95% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса
28.60 kDa
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Interferon gamma receptor 1 (CD119/IFNGR1) is a ~54 kDa protein. Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Associates with transmembrane accessory factor IFNGR2 to form a functional receptor. Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to dimerization, translocation to the nucleus, and stimulation of target gene transcription.
1. van de Wetering, D. et al. (2010) Molecular immunology 47, 1023-30. PMID: 20015550
2. Thiel, DJ. et al. (2000) Structure (London, England : 1993) 8, 927-36. PMID: 10986460
3. Aguet, M. et al. (1988) Cell 55, 273-80. PMID: 2971451
4. Sakatsume, M. et al. (1995) The Journal of biological chemistry 270, 17528-34. PMID: 7615558
5. Walter, MR. et al. (1995) Nature 376, 230-5. PMID: 7617032
6. Kotenko, SV. et al. (1995) The Journal of biological chemistry 270, 20915-21. PMID: 7673114
7. Londino, JD. et al. (2017) The Biochemical journal 474, 3543-3557. PMID: 28883123
Примечание
For research use only.
Изображения
SDS-PAGE
SDS-PAGE for Recombinant Human CD119/IFNGR1 Protein, C-His
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