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| カタログ番号 | HB732011 | ||||||
|---|---|---|---|---|---|---|---|
| 説明 |
Recombinant Human CD124/IL4R Protein, C-His (HB732011) expressed in Mammalian Cells, spanning Met1-His232. Purity: >90% by SDS-PAGE. Highlights
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| 発現システム | Mammalian cells | ||||||
| アクセッション番号 | P24394 | ||||||
| タンパク質長 | Met1-His232 | ||||||
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress | ||||||
| 種 | Homo sapiens (Human) | ||||||
| 性質 | Recombinant | ||||||
| ターゲット | CD124, IL-4 receptor subunit alpha, IL-4-binding protein, IL-4R subunit alpha, IL-4R-alpha, IL-4RA, IL4-BP, IL4R, IL4RA, Interleukin-4 receptor subunit alpha, Soluble IL-4 receptor subunit alpha, Soluble IL-4R-alpha, Soluble interleukin-4 receptor subunit alpha, sIL4Ralpha/prot | ||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||
| 純度 | >90% as determined by SDS-PAGE. | ||||||
| 予測分子量 | 27.26 kDa | ||||||
| 形態 | Lyophilized | ||||||
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
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| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. | ||||||
| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||
| 別名 | CD124, IL-4 receptor subunit alpha, IL-4-binding protein, IL-4R subunit alpha, IL-4R-alpha, IL-4RA, IL4-BP, IL4R, IL4RA, Interleukin-4 receptor subunit alpha, Soluble IL-4 receptor subunit alpha, Soluble IL-4R-alpha, Soluble interleukin-4 receptor subunit alpha, sIL4Ralpha/prot | ||||||
| 背景 | Interleukin-4 receptor subunit alpha (CD124/IL4R) is a ~89 kDa protein. Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. CD124 is the therapeutic target of dupilumab (Dupixent). 1. Andrews, AL. et al. (2006) The Journal of allergy and clinical immunology 118, 858-65. PMID: 17030238 | ||||||
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CD124/IL4R Protein, C-His
Detected by Research Grade Rademikibart (Catalog No: HB732136) in indirect ELISA
Detected by Stapokibart (Catalog No: HB732146) in indirect ELISA.
Detected by MEDI2045 (Catalog No: HB732106) in indirect ELISA.
Immobilized HB732011 Human CD124/IL4R Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HY457011 Human IL4 Recombinant Protein (N-Fc), the EC 50 is 16.5 ng/mL.
Immobilized HB732011 Human CD124/IL4R Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HY457031 Human IL4 Recombinant Protein (N-mFc), the EC 50 is 68.8 ng/mL.
Immobilized HY457011 Human IL4 Recombinant Protein (N-Fc) at 2 μg/mL(100 μL/well) can bind HB732011 Human CD124/IL4R Recombinant Protein (C-His), the EC 50 is 10.2 ng/mL.
Immobilized HY457031 Human IL4 Recombinant Protein (N-mFc) at 2 μg/mL(100 μL/well) can bind HB732011 Human CD124/IL4R Recombinant Protein (C-His), the EC 50 is 23.3 ng/mL.
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