Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Monocyte differentiation antigen CD14 is a ~40 kDa protein. Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-).
1. Wright, SD. et al. (1990) Science (New York, N.Y.) 249, 1431-3. PMID: 1698311
2. Kelley, SL. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 190, 1304-11. PMID: 23264655
3. Tsukamoto, H. et al. (2010) International immunology 22, 271-80. PMID: 20133493
4. Murakami, K. et al. (2012) FEBS letters 586, 319-24. PMID: 22265692
5. Haziot, A. et al. (1996) Immunity 4, 407-14. PMID: 8612135
6. Triantafilou, M. et al. (2006) The Journal of biological chemistry 281, 31002-11. PMID: 16880211
7. Estruch, M. et al. (2013) Atherosclerosis 229, 356-62. PMID: 23880187
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human CD14 Protein, N-His
Western blot
Detected by Anti-CD14 monoclonal antibody (Catalog No: HY373026)
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