Recombinant Human CD177 Protein, C-His (HV109011) expressed in Mammalian Cells, spanning Met1-Gly408. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression
Mammalian cells
Numéro d'accès
Q8N6Q3
Longueur de protéine
Met1-Gly408
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces
Homo sapiens (Human)
Nature
Recombinant
Niveau d'endotoxines
Please contact with the lab for this information.
Pureté
>90% as determined by SDS-PAGE.
Masse moléculaire prédite
44.37 kDa
Forme
Lyophilized
Tampon de stockage
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs
CD177, NB1 GP, HNA-2a, Human neutrophil alloantigen 2a, NB1, PRV-1, CD177 antigen, Polycythemia rubra vera protein 1, NB1 glycoprotein, PRV1
Contexte
CD177 antigen is a ~46 kDa protein. In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF primed neutrophils including degranulation and superoxide production. In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration. Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro. However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo. Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils.
1. Jerke, U. et al. (2011) The Journal of biological chemistry 286, 7070-81. PMID: 21193407
2. Bai, M. et al. (2017) Blood 130, 2092-2100. PMID: 28807980
3. Sachs, UJ. et al. (2007) The Journal of biological chemistry 282, 23603-12. PMID: 17580308
4. Wang, L. et al. (2013) European journal of haematology 90, 436-7. PMID: 23461681
5. von Vietinghoff, S. et al. (2007) Blood 109, 4487-93. PMID: 17244676
6. von Vietinghoff, S. et al. (2008) Clinical and experimental immunology 152, 508-16. PMID: 18462208
7. Kuckleburg, CJ. et al. (2013) Arteriosclerosis, thrombosis, and vascular biology 33, 275-84. PMID: 23202369
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human CD177 Protein, C-His
中文
English
한국어
日本語
Español
Français
Русский
