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| カタログ番号 | HV109012 |
|---|---|
| 説明 |
Recombinant Human CD177 Protein, N-His (HV109012) expressed in E. coli, spanning Cys133-Ser301. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q8N6Q3 |
| タンパク質長 | Cys133-Ser301 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 20.24 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | CD177, NB1 GP, HNA-2a, Human neutrophil alloantigen 2a, NB1, PRV-1, CD177 antigen, Polycythemia rubra vera protein 1, NB1 glycoprotein, PRV1 |
| 背景 | CD177 antigen is a ~46 kDa protein. In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF primed neutrophils including degranulation and superoxide production. In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration. Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro. However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo. Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils. 1. Jerke, U. et al. (2011) The Journal of biological chemistry 286, 7070-81. PMID: 21193407 2. Bai, M. et al. (2017) Blood 130, 2092-2100. PMID: 28807980 3. Sachs, UJ. et al. (2007) The Journal of biological chemistry 282, 23603-12. PMID: 17580308 4. Wang, L. et al. (2013) European journal of haematology 90, 436-7. PMID: 23461681 5. von Vietinghoff, S. et al. (2007) Blood 109, 4487-93. PMID: 17244676 6. von Vietinghoff, S. et al. (2008) Clinical and experimental immunology 152, 508-16. PMID: 18462208 7. Kuckleburg, CJ. et al. (2013) Arteriosclerosis, thrombosis, and vascular biology 33, 275-84. PMID: 23202369 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CD177 Protein, N-His
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
