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| カタログ番号 | HX206022 |
|---|---|
| 説明 |
Recombinant Human CD184/CXCR4 Protein, N-GST & C-His (HX206022) expressed in E. coli. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P61073 |
| タンパク質長 | Glu2-Lys38&Gly306-Ser352 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 37.40 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | C-X-C chemokine receptor type 4, CXC-R4, CXCR-4, FB22, Fusin, HM89, LCR1, Leukocyte-derived seven transmembrane domain receptor, LESTR, Lipopolysaccharide-associated protein 3, LAP-3, LPS-associated protein 3, NPYRL, Stromal cell-derived factor 1 receptor, SDF-1 receptor, CD184, CXCR4 |
| 背景 | C-X-C chemokine receptor type 4 (CD184/CXCR4) is a ~39 kDa protein. Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. CXCR4 is coupled to G(i) G alpha proteins and mediates inhibition of adenylate cyclase. Involved in the AKT signaling cascade. Plays a role in regulation of cell migration, e.g. 1. Brelot, A. et al. (1999) Journal of virology 73, 2576-86. PMID: 10074102 2. Gupta, SK. et al. (1999) Journal of immunology (Baltimore, Md. : 1950) 163, 2368-72. PMID: 10452968 3. Cheng, ZJ. et al. (2000) The Journal of biological chemistry 275, 2479-85. PMID: 10644702 4. Brelot, A. et al. (2000) The Journal of biological chemistry 275, 23736-44. PMID: 10825158 5. Veldkamp, CT. et al. (2008) Science signaling 1, ra4. PMID: 18799424 6. Busillo, JM. et al. (2010) The Journal of biological chemistry 285, 7805-17. PMID: 20048153 7. Malik, R. et al. (2010) Molecular biology of the cell 21, 2529-41. PMID: 20505072 8. Cao, Y. et al. (2015) Leukemia 29, 169-76. PMID: 24912431 9. Lear, T. et al. (2017) American journal of physiology. Cell physiology 313, C584-C592. PMID: 28978524 10. Bleul, CC. et al. (1996) Nature 382, 829-33. PMID: 8752280 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CD184/CXCR4 Protein, N-GST & C-His
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