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| カタログ番号 | HV427012 |
|---|---|
| 説明 |
Recombinant Human CD246/ALK Protein, N-His (HV427012) expressed in E. coli, spanning Met1348-Ser1611. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9UM73 |
| タンパク質長 | Met1348-Ser1611 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 31.03 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | ALK, ALK tyrosine kinase receptor, CD246, Anaplastic lymphoma kinase |
| 背景 | ALK tyrosine kinase receptor (CD246) is a ~176 kDa protein. Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Also acts as a key thinness protein involved in the resistance to weight gain: in hypothalamic neurons, controls energy expenditure acting as a negative regulator of white adipose tissue lipolysis and sympathetic tone to fine-tune energy homeostasis. Following activation by ALKAL2 ligand at the cell surface, transduces an extracellular signal into an intracellular response. In contrast, ALKAL1 is not a potent physiological ligand for ALK. Ligand-binding to the extracellular domain induces tyrosine kinase activation, leading to activation of the mitogen-activated protein kinase (MAPK) pathway. 1. Souttou, B. et al. (2001) The Journal of biological chemistry 276, 9526-31. PMID: 11121404 2. Simonitsch, I. et al. (2001) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 15, 1416-8. PMID: 11387242 3. Gouzi, JY. et al. (2005) Journal of cell science 118, 5811-23. PMID: 16317043 4. Degoutin, J. et al. (2007) FEBS letters 581, 727-34. PMID: 17274988 5. Reshetnyak, AV. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 8340-8345. PMID: 30061385 6. De Munck, S. et al. (2021) Nature 600, 143-147. PMID: 34646012 7. Reshetnyak, AV. et al. (2021) Nature 600, 153-157. PMID: 34819673 8. Borenäs, M. et al. (2021) The EMBO journal 40, e105784. PMID: 33411331 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CD246/ALK Protein, N-His
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