Human CD27 Ligand/TNFSF7/CD70 Recombinant Protein (C-Fc) (HB796011)

Name: Human CD27 Ligand/TNFSF7/CD70 Recombinant Protein (C-Fc)
Brand: abinScience
SKU: HB796011
Price: 298 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue	HB796011
Description	Recombinant Human CD27 Protein, C-Fc (HB796011) expressed in Mammalian Cells, spanning Met1-Ile192. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	Mammalian cells
Numéro d'accès	P26842
Longueur de protéine	Met1-Ile192
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Applications testées	WB
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>95% as determined by SDS-PAGE.
Masse moléculaire prédite	47.84 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	CD27L receptor, TNFRSF7, T14, CD27 antigen, Tumor necrosis factor receptor superfamily member 7, CD27, T-cell activation antigen CD27
Contexte	CD27 antigen is a ~29 kDa protein. Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells. Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory. In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis. Negatively regulates the function of Th2 lymphocytes in the adipose tissue. 1. Izawa, K. et al. (2017) The Journal of experimental medicine 214, 73-89. PMID: 28011863 2. Jaeger-Ruckstuhl, CA. et al. (2024) Immunity 57, 287-302.e12. PMID: 38354704
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human CD27 Protein, C-Fc
Bioactivity

Detects Varlilumab in indirect ELISAs.
Bioactivity

Detects Human CD27 Antibody (5F24) in indirect ELISAs.
Western blot

Detected by Anti-CD27 monoclonal antibody (Catalog No: HP628207)
Bioactivity

Immobilized HB796011 Human CD27 Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HW717011 Human CD70 Recombinant Protein (N-Fc), the EC 50 is 5.78 μg/mL.
Bioactivity

Immobilized MW717021 Mouse CD70 Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HB796011 Human CD27 Recombinant Protein (C-Fc), the EC 50 is 310 ng/mL.