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| カタログ番号 | HC341011 |
|---|---|
| 説明 |
Recombinant Human CD317/BST2 Protein, C-His (HC341011) expressed in Mammalian Cells, spanning Asn49-Ser160. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | Mammalian cells |
| アクセッション番号 | Q10589 |
| タンパク質長 | Asn49-Ser160 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 15.86 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Tetherin, Bone marrow stromal antigen 2, BST2, BST-2, CD317, HM1.24 antigen |
| 背景 | Bone marrow stromal antigen 2 (CD317/BST2) is a ~19 kDa protein. IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. Acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted. Its target viruses belong to diverse families, including retroviridae: human immunodeficiency virus type 1 (HIV-1), human immunodeficiency virus type 2 (HIV-2), simian immunodeficiency viruses (SIVs), equine infectious anemia virus (EIAV), feline immunodeficiency virus (FIV), prototype foamy virus (PFV), Mason-Pfizer monkey virus (MPMV), human T-cell leukemia virus type 1 (HTLV-1), Rous sarcoma virus (RSV) and murine leukemia virus (MLV), flavivirideae: hepatitis C virus (HCV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), arenaviridae: lassa virus (LASV) and machupo virus (MACV), herpesviridae: kaposis sarcoma-associated herpesvirus (KSHV), rhabdoviridae: vesicular stomatitis virus (VSV), orthomyxoviridae: influenza A virus, paramyxoviridae: nipah virus, and coronaviridae: SARS-CoV. 1. Neil, SJ. et al. (2008) Nature 451, 425-30. PMID: 18200009 2. Van Damme, N. et al. (2008) Cell host & microbe 3, 245-52. PMID: 18342597 3. Jouvenet, N. et al. (2009) Journal of virology 83, 1837-44. PMID: 19036818 4. Perez-Caballero, D. et al. (2009) Cell 139, 499-511. PMID: 19879838 5. Miyakawa, K. et al. (2009) PLoS pathogens 5, e1000700. PMID: 20019814 6. Hinz, A. et al. (2010) Cell host & microbe 7, 314-323. PMID: 20399176 7. Pardieu, C. et al. (2010) PLoS pathogens 6, e1000843. PMID: 20419159 8. Yang, H. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 18428-32. PMID: 20940320 9. Xu, F. et al. (2011) Virology journal 8, 198. PMID: 21529378 10. Dafa-Berger, A. et al. (2012) Virology 428, 98-111. PMID: 22520941 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CD317/BST2 Protein, C-His
Detects Human CD317/BST2 Antibody (HM1.24) in indirect ELISAs.
Detected by Anti-CD317/BST2 monoclonal antibody (Catalog No: HC341107)
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