Recombinant Human CD366/HAVCR2/TIM-3 Protein, C-His (HV029011) expressed in Mammalian Cells, spanning Met1-Arg200.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
Система экспрессии
Mammalian cells
Номер доступа
Q8TDQ0
Длина белка
Met1-Arg200
Применения
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Homo sapiens (Human)
Характер
Recombinant
Цель
CD366, HAVCR2, HAVcr-2, Hepatitis A virus cellular receptor 2, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIM-3, TIM3, TIMD-3, TIMD3
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>85% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса
23.25 kDa
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия
CD366, HAVCR2, HAVcr-2, Hepatitis A virus cellular receptor 2, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIM-3, TIM3, TIMD-3, TIMD3
Фон
Hepatitis A virus cellular receptor 2 (CD366/TIM-3) is a ~33 kDa protein. Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand. Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance.
1. Gorman, JV. et al. (2014) Immunologic research 59, 56-65. PMID: 24825777
2. Monney, L. et al. (2002) Nature 415, 536-41. PMID: 11823861
3. Sánchez-Fueyo, A. et al. (2003) Nature immunology 4, 1093-101. PMID: 14556005
4. Clayton, KL. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 192, 782-91. PMID: 24337741
5. Tomkowicz, B. et al. (2015) PloS one 10, e0140694. PMID: 26492563
6. Gautron, AS. et al. (2014) European journal of immunology 44, 2703-2711. PMID: 24838857
7. Zhu, C. et al. (2005) Nature immunology 6, 1245-52. PMID: 16286920
8. Leitner, J. et al. (2013) PLoS pathogens 9, e1003253. PMID: 23555261
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