Human CD4 Recombinant Protein (C-His) (HF998011)

Name: Human CD4 Recombinant Protein (C-His)
Brand: abinScience
SKU: HF998011
Price: 298 USD
Availability: InStock

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50ug
100ug
1mg

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Datasheet
SDS

Vista general

Número de catálogo	HF998011
Descripción	Recombinant Human CD4 Protein, C-His (HF998011) expressed in Mammalian Cells, spanning Met1-Trp390. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	Mammalian cells
Número de acceso	P01730
Longitud de proteína	Met1-Trp390
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Aplicaciones probadas	WB
Objetivo	CD4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	44.36 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	CD4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Fondo	T-cell surface glycoprotein CD4 is a ~51 kDa protein. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human CD4 Protein, C-His
Western blot

Detected by Anti-CD4 monoclonal antibody (Catalog No: HF998107)
Western blot

Detected by Anti-CD4 monoclonal antibody (Catalog No: HF998207)
Western blot

Detected by Anti-CD4 monoclonal antibody (Catalog No: HF998036)
Western blot

Detected by Anti-CD4 monoclonal antibody (Catalog No: HY393023)
Bioactivity

Detected by Anti-Human CD4 Antibody (OKT4A) (Catalog No: HF998307) in indirect ELISA
Bioactivity

Detected by Semzuvolimab (Catalog No: HF998086) in indirect ELISA.