Recombinant Human CD47/MER6 Protein, C-His (HC359011) expressed in Mammalian Cells, spanning Met1-Glu141.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression
Mammalian cells
Numéro d'accès
Q08722
Longueur de protéine
Met1-Glu141
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces
Homo sapiens (Human)
Nature
Recombinant
Cible
Antigenic surface determinant protein OA3, CD47, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, Protein MER6
Niveau d'endotoxines
Please contact with the lab for this information.
Pureté
>95% as determined by SDS-PAGE.
Masse moléculaire prédite
16.68 kDa
Forme
Lyophilized
Tampon de stockage
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs
Antigenic surface determinant protein OA3, CD47, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, Protein MER6
Contexte
Leukocyte surface antigen CD47 (MER6) is a ~35 kDa protein. Adhesive protein that mediates cell-to-cell interactions. Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins. Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Plays a role in modulating pulmonary endothelin EDN1 signaling. Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure. CD47 is the therapeutic target of magrolimab (clinical).
1. Latour, S. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 2547-54. PMID: 11509594
2. Piccio, L. et al. (2005) Blood 105, 2421-7. PMID: 15383453
3. Isenberg, JS. et al. (2009) The Journal of biological chemistry 284, 1116-25. PMID: 19004835
4. Lindberg, FP. et al. (1993) The Journal of cell biology 123, 485-96. PMID: 7691831
5. Gao, AG. et al. (1996) The Journal of biological chemistry 271, 21-4. PMID: 8550562
6. Rogers, NM. et al. (2017) Cardiovascular research 113, 15-29. PMID: 27742621
SDS-PAGE for Recombinant Human CD47/MER6 Protein, C-His
Bioactivity
Detects Lemzoparlimab in indirect ELISAs.
Bioactivity
Detects Human CD47/MER6 Antibody (B6H12) in indirect ELISAs.
Bioactivity
Detects Ligufalimab in indirect ELISAs.
Bioactivity
Detects Human CD47/MER6(CC-90002) in indirect ELISAs.
Bioactivity
Detected by Research Grade Letaplimab (Catalog No: HC359046) in indirect ELISA
Bioactivity
Detected by Zeripatamig (Catalog No: HC359346) in indirect ELISA.
Bioactivity
Detected by Amulirafusp Alfa (Catalog No: HC359376) in indirect ELISA.
Bioactivity
Immobilized HC359011 Human CD47 Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HX892021 Human CD172a/SIRPA Recombinant Protein (C-Fc), the EC 50 is 402 ng/mL.
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