Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Cell adhesion molecule CEACAM5 (CD66e) is a ~76 kDa protein. Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells.
1. Taheri, M. et al. (2000) The Journal of biological chemistry 275, 26935-43. PMID: 10864933
2. Ordoñez, C. et al. (2000) Cancer research 60, 3419-24. PMID: 10910050
3. Oikawa, S. et al. (1989) Biochemical and biophysical research communications 164, 39-45. PMID: 2803308
Примечание
For research use only.
Изображения
SDS-PAGE
SDS-PAGE for Recombinant Human CD66e/CEA/CEACAM5 Protein, C-His
Bioactivity
Detects cT84.66 in indirect ELISAs.
Bioactivity
Detected by Research Grade Altumomab (Cat No.: HY546046) inindirectELISA.
Bioactivity
Detected by Research Grade Anti-Human CD66e/CEA/CEACAM5 (SGM 101) (Cat No.: HY546106) inindirectELISA.
Western blot
Detected by Anti-CD66e/CEA/CEACAM5 monoclonal antibody (Catalog No: HY546106)
Western blot
Detected by Anti-CD66e/CEA/CEACAM5 monoclonal antibody (Catalog No: HY546046)
Western blot
Detected by Anti-CD66e/CEA/CEACAM5 monoclonal antibody (Catalog No: HT246016)
Bioactivity
Detected by C43 (Catalog No: HY546063) in indirect ELISA.
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