Recombinant Human CD85k/LILRB4/ILT3 Protein, C-His (HV126011) expressed in Mammalian Cells, spanning Met1-Glu259. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression
Mammalian cells
Numéro d'accès
Q8NHJ6
Longueur de protéine
Met1-Glu259
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces
Homo sapiens (Human)
Nature
Recombinant
Niveau d'endotoxines
Please contact with the lab for this information.
Pureté
>90% as determined by SDS-PAGE.
Masse moléculaire prédite
29.47 kDa
Forme
Lyophilized
Tampon de stockage
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs
CD85 antigen-like family member K, LIR-5, Leukocyte immunoglobulin-like receptor 5, ILT3, ILT-3, Immunoglobulin-like transcript 3, LILRB4, CD85k, Leukocyte immunoglobulin-like receptor subfamily B member 4, Monocyte inhibitory receptor HM18, LIR5
Contexte
Leukocyte immunoglobulin-like receptor subfamily B member 4 (CD85k/LILRB4) is a ~49 kDa protein. Inhibitory receptor involved in the down-regulation of the immune response and the development of immune tolerance. Receptor for FN1. Receptor for apolipoprotein APOE. Receptor for ALCAM/CD166. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
1. Chang, CC. et al. (2002) Nature immunology 3, 237-43. PMID: 11875462
2. Su, MT. et al. (2021) International immunology 33, 447-458. PMID: 34089617
3. Deng, M. et al. (2018) Nature 562, 605-609. PMID: 30333625
4. Xu, Z. et al. (2018) Journal of immunology (Baltimore, Md. : 1950) 200, 1207-1219. PMID: 29263213
5. Cella, M. et al. (1997) The Journal of experimental medicine 185, 1743-51. PMID: 9151699
6. Lu, HK. et al. (2009) The Journal of biological chemistry 284, 34839-48. PMID: 19833736
7. Kim-Schulze, S. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 176, 2790-8. PMID: 16493035
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human CD85k/LILRB4/ILT3 Protein, C-His
Bioactivity
Detects Human CD85k/LILRB4 Antibody (128-3#) in indirect ELISAs.
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