

| Numéro de catalogue | HD509012 |
|---|---|
| Description |
Recombinant Human CDT1 Protein, N-His (HD509012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| Système d'expression | E. coli |
| Numéro d'accès | Q9H211 |
| Longueur de protéine | Thr112-Ser386 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Espèces | Homo sapiens (Human) |
| Nature | Recombinant |
| Niveau d'endotoxines | Please contact with the lab for this information. |
| Pureté | >90% as determined by SDS-PAGE. |
| Forme | Lyophilized |
| Tampon de stockage | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Expédition | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stabilité et stockage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Noms alternatifs | CDT1, DNA replication factor Cdt1, DUP, Double parked homolog |
| Contexte | DNA replication factor Cdt1 is a ~60 kDa protein. Required for both DNA replication and mitosis. DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC). Required also for mitosis by promoting stable kinetochore-microtubule attachments. Potential oncogene. 1. Wohlschlegel, JA. et al. (2000) Science (New York, N.Y.) 290, 2309-12. PMID: 11125146 2. Sugimoto, N. et al. (2004) The Journal of biological chemistry 279, 19691-7. PMID: 14993212 3. Miotto, B. et al. (2011) Molecular cell 44, 62-71. PMID: 21856198 4. Varma, D. et al. (2012) Nature cell biology 14, 593-603. PMID: 22581055 5. Franz, A. et al. (2016) Nature communications 7, 10612. PMID: 26842564 6. Cook, JG. et al. (2004) The Journal of biological chemistry 279, 9625-33. PMID: 14672932 |
| Note | For research use only |
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