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Human CDT1 Recombinant Protein (N-His) (HD509012)

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  • 1mg
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Aperçu
Numéro de catalogueHD509012
Description
Recombinant Human CDT1 Protein, N-His (HD509012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
Système d'expressionE. coli
Numéro d'accèsQ9H211
Longueur de protéineThr112-Ser386
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsCDT1, DNA replication factor Cdt1, DUP, Double parked homolog
Contexte

DNA replication factor Cdt1 is a ~60 kDa protein. Required for both DNA replication and mitosis. DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC). Required also for mitosis by promoting stable kinetochore-microtubule attachments. Potential oncogene.

1. Wohlschlegel, JA. et al. (2000) Science (New York, N.Y.) 290, 2309-12. PMID: 11125146
2. Sugimoto, N. et al. (2004) The Journal of biological chemistry 279, 19691-7. PMID: 14993212
3. Miotto, B. et al. (2011) Molecular cell 44, 62-71. PMID: 21856198
4. Varma, D. et al. (2012) Nature cell biology 14, 593-603. PMID: 22581055
5. Franz, A. et al. (2016) Nature communications 7, 10612. PMID: 26842564
6. Cook, JG. et al. (2004) The Journal of biological chemistry 279, 9625-33. PMID: 14672932
Note For research use only
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Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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