Anti-Human CDT1 Polyclonal Antibody (HD509014)

Name: Anti-Human CDT1 Polyclonal Antibody
Brand: abinScience
SKU: HD509014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HD509014

Description

Anti-Human CDT1 Polyclonal Antibody (HD509014) is a rabbit polyclonal antibody detecting CDT1. Suitable for Human.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CDT1 (Thr112-Ser386).

Target

CDT1, DNA replication factor Cdt1, DUP, Double parked homolog

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q9H211

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

DNA replication factor Cdt1 is a ~60 kDa protein. Required for both DNA replication and mitosis. DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC). Required also for mitosis by promoting stable kinetochore-microtubule attachments. Potential oncogene.

1. Wohlschlegel, JA. et al. (2000) Science (New York, N.Y.) 290, 2309-12. PMID: 11125146

2. Sugimoto, N. et al. (2004) The Journal of biological chemistry 279, 19691-7. PMID: 14993212

3. Miotto, B. et al. (2011) Molecular cell 44, 62-71. PMID: 21856198

4. Varma, D. et al. (2012) Nature cell biology 14, 593-603. PMID: 22581055

5. Franz, A. et al. (2016) Nature communications 7, 10612. PMID: 26842564

6. Cook, JG. et al. (2004) The Journal of biological chemistry 279, 9625-33. PMID: 14672932

Note

For research use only

Images

Western blot

Lane 1: HCT116 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
Lane 4: MCF-7 cell lysate
Lane 5: Hela cell lysate

References

Datasheet

Anti-Human CDT1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CDT1 Polyclonal Antibody (HD509014)

Overview

Images

References

Datasheet

SDS

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