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Human CENPJ Recombinant Protein (N-His) (HP186022)

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Aperçu
Numéro de catalogueHP186022
Description
Recombinant Human CENPJ Protein, N-His (HP186022) expressed in E. coli, spanning Ile1149-Leu1338. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expressionE. coli
Numéro d'accèsQ9HC77
Longueur de protéineIle1149-Leu1338
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Masse moléculaire prédite 23.99 kDa
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsCENP-J, CENPJ, CPAP, Centromere protein J, Centrosomal P4.1-associated protein, Centrosome assembly and centriole elongation protein, LAG-3-associated protein, LAP, LIP1, LYST-interacting protein 1
Contexte

Centrosomal P4.1-associated protein is a ~153 kDa protein. Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome. Involved in the regulation of slow processive growth of centriolar microtubules. Acts as a microtubule plus-end tracking protein that stabilizes centriolar microtubules and inhibits microtubule polymerization and extension from the distal ends of centrioles. Required for centriole elongation and for STIL-mediated centriole amplification.

1. Kleylein-Sohn, J. et al. (2007) Developmental cell 13, 190-202. PMID: 17681131
2. Chang, J. et al. (2010) The EMBO journal 29, 2395-406. PMID: 20531387
3. Hung, LY. et al. (2004) Molecular biology of the cell 15, 2697-706. PMID: 15047868
4. Sharma, A. et al. (2016) Developmental cell 37, 362-376. PMID: 27219064
5. Zheng, X. et al. (2016) Nature communications 7, 11874. PMID: 27306797
6. Tang, CJ. et al. (2011) The EMBO journal 30, 4790-804. PMID: 22020124
7. Chang, CW. et al. (2016) Journal of cell science 129, 2501-13. PMID: 27185865
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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