Human CENPJ Recombinant Protein (N-His) (HP186012)

Name: Human CENPJ Recombinant Protein (N-His)
Brand: abinScience
SKU: HP186012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP186012
Description	Recombinant Human CENPJ Protein, N-His (HP186012) expressed in E. coli, spanning Met1-Asn207. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9HC77
Protein length	Met1-Asn207
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	25.72 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	LYST-interacting protein 1, CPAP, LAP, LAG-3-associated protein, LIP1, Centrosomal P4.1-associated protein, CENPJ, Centromere protein J, CENP-J
Background	Centrosomal P4.1-associated protein (CENPJ) is a ~153 kDa protein. Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome. Involved in the regulation of slow processive growth of centriolar microtubules. Acts as a microtubule plus-end tracking protein that stabilizes centriolar microtubules and inhibits microtubule polymerization and extension from the distal ends of centrioles. Required for centriole elongation and for STIL-mediated centriole amplification. 1. Kleylein-Sohn, J. et al. (2007) Developmental cell 13, 190-202. PMID: 17681131 2. Chang, J. et al. (2010) The EMBO journal 29, 2395-406. PMID: 20531387 3. Hung, LY. et al. (2004) Molecular biology of the cell 15, 2697-706. PMID: 15047868 4. Sharma, A. et al. (2016) Developmental cell 37, 362-376. PMID: 27219064 5. Zheng, X. et al. (2016) Nature communications 7, 11874. PMID: 27306797 6. Tang, CJ. et al. (2011) The EMBO journal 30, 4790-804. PMID: 22020124 7. Chang, CW. et al. (2016) Journal of cell science 129, 2501-13. PMID: 27185865
Note	For research use only.

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SDS-PAGE for Recombinant Human CENPJ Protein, N-His

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Datasheet

Human CENPJ Recombinant Protein (N-His).pdf

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Human CENPJ Recombinant Protein (N-His) (HP186012)

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