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Human CENPJ Recombinant Protein (N-His) (HP186022)

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Overview
Catalog No.HP186022
Description
Recombinant Human CENPJ Protein, N-His (HP186022) expressed in E. coli, spanning Ile1149-Leu1338. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionQ9HC77
Protein lengthIle1149-Leu1338
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 23.99 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesCENP-J, CENPJ, CPAP, Centromere protein J, Centrosomal P4.1-associated protein, Centrosome assembly and centriole elongation protein, LAG-3-associated protein, LAP, LIP1, LYST-interacting protein 1
Background

Centrosomal P4.1-associated protein is a ~153 kDa protein. Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome. Involved in the regulation of slow processive growth of centriolar microtubules. Acts as a microtubule plus-end tracking protein that stabilizes centriolar microtubules and inhibits microtubule polymerization and extension from the distal ends of centrioles. Required for centriole elongation and for STIL-mediated centriole amplification.

1. Kleylein-Sohn, J. et al. (2007) Developmental cell 13, 190-202. PMID: 17681131
2. Chang, J. et al. (2010) The EMBO journal 29, 2395-406. PMID: 20531387
3. Hung, LY. et al. (2004) Molecular biology of the cell 15, 2697-706. PMID: 15047868
4. Sharma, A. et al. (2016) Developmental cell 37, 362-376. PMID: 27219064
5. Zheng, X. et al. (2016) Nature communications 7, 11874. PMID: 27306797
6. Tang, CJ. et al. (2011) The EMBO journal 30, 4790-804. PMID: 22020124
7. Chang, CW. et al. (2016) Journal of cell science 129, 2501-13. PMID: 27185865
Note For research use only
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Formula
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