中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HY223012 |
|---|---|
| 説明 |
Recombinant Human CFI Protein, N-His (HY223012) expressed in E. coli, spanning Lys19-Ile335. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P05156 |
| タンパク質長 | Lys19-Ile335 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 37.58 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Complement factor I, CFI, C3B/C4B inactivator, IF |
| 背景 | Complement factor I (CFI) is a ~65 kDa protein. Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins. Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces. The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization. 1. Timár, KK. et al. (2007) Molecular immunology 44, 2943-9. PMID: 17320177 2. Harrison, RA. et al. (1980) Molecular immunology 17, 9-20. PMID: 7360115 3. Barilla-LaBarca, ML. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 6298-304. PMID: 12055245 4. Seya, T. et al. (1990) Journal of biochemistry 107, 310-5. PMID: 2141838 5. Sahu, A. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 160, 5596-604. PMID: 9605165 6. Xue, X. et al. (2017) Nature structural & molecular biology 24, 643-651. PMID: 28671664 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CFI Protein, N-His
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
