Anti-CFI Polyclonal Antibody (HY223014)

Name: Anti-CFI Polyclonal Antibody
Brand: abinScience
SKU: HY223014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HY223014

Description

Anti-CFI Polyclonal Antibody (HY223014) is a rabbit polyclonal antibody detecting CFI in ELISA, IHC, WB. Suitable for Human and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CFI (Lys19-Ile335).

Target

Complement factor I, CFI, C3B/C4B inactivator, IF

Purification

Purified by antigen affinity column.

Accession

P05156

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Complement factor I (CFI) is a ~65 kDa protein. Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins. Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces. The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization.

1. Timár, KK. et al. (2007) Molecular immunology 44, 2943-9. PMID: 17320177

2. Harrison, RA. et al. (1980) Molecular immunology 17, 9-20. PMID: 7360115

3. Barilla-LaBarca, ML. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 6298-304. PMID: 12055245

4. Seya, T. et al. (1990) Journal of biochemistry 107, 310-5. PMID: 2141838

5. Sahu, A. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 160, 5596-604. PMID: 9605165

6. Xue, X. et al. (2017) Nature structural & molecular biology 24, 643-651. PMID: 28671664

Note

For research use only.

Images

References

Datasheet

Anti-CFI Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-CFI Polyclonal Antibody (HY223014)

Overview

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References

Datasheet

SDS

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