Human CHEK1 Recombinant Protein (N-His) (HA503012)

Name: Human CHEK1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA503012
Price: 211 USD
Availability: InStock

Datasheet Compartir producto Copiar información

Precio(USD):

Especificación:

50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo	HA503012
Descripción	Recombinant Human CHEK1 Protein, N-His (HA503012) expressed in E. coli, spanning Met1-Asn266. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	O14757
Longitud de proteína	Met1-Asn266
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	32.99 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Serine/threonine-protein kinase Chk1, 2.7.11.1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1
Fondo	Serine/threonine-protein kinase Chk1 is a ~54 kDa protein. Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. 1. Feijoo, C. et al. (2001) The Journal of cell biology 154, 913-23. PMID: 11535615 2. Zhao, H. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 14795-800. PMID: 12399544 3. Heffernan, TP. et al. (2002) Molecular and cellular biology 22, 8552-61. PMID: 12446774 4. Chen, MS. et al. (2003) Molecular and cellular biology 23, 7488-97. PMID: 14559997 5. Pichierri, P. et al. (2004) The EMBO journal 23, 1178-87. PMID: 14988723 6. Krämer, A. et al. (2004) Nature cell biology 6, 884-91. PMID: 15311285 7. Huang, X. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 1065-70. PMID: 15650047 8. Sørensen, CS. et al. (2005) Nature cell biology 7, 195-201. PMID: 15665856 9. Yu, X. et al. (2020) Journal of hematology & oncology 13, 40. PMID: 32357935
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human CHEK1 Protein, N-His

Referencias

Datasheet

Human CHEK1 Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo