Anti-Human CHEK1 Polyclonal Antibody (HA503014)

Name: Anti-Human CHEK1 Polyclonal Antibody
Brand: abinScience
SKU: HA503014
Price: 96 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HA503014

Description

Anti-Human CHEK1 Polyclonal Antibody (HA503014) is a rabbit polyclonal antibody detecting CHEK1 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Gallus gallus, Mus musculus, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human CHEK1 (Met1-Asn266).

Target

CHEK1

Purification

Purified by antigen affinity column.

Accession

O14757

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Serine/threonine-protein kinase Chk1, 2.7.11.1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1

Background

Serine/threonine-protein kinase Chk1 (CHEK1) is a ~54 kDa protein. Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C.

1. Feijoo, C. et al. (2001) The Journal of cell biology 154, 913-23. PMID: 11535615

2. Zhao, H. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 14795-800. PMID: 12399544

3. Heffernan, TP. et al. (2002) Molecular and cellular biology 22, 8552-61. PMID: 12446774

4. Chen, MS. et al. (2003) Molecular and cellular biology 23, 7488-97. PMID: 14559997

5. Pichierri, P. et al. (2004) The EMBO journal 23, 1178-87. PMID: 14988723

6. Krämer, A. et al. (2004) Nature cell biology 6, 884-91. PMID: 15311285

7. Huang, X. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 1065-70. PMID: 15650047

8. Sørensen, CS. et al. (2005) Nature cell biology 7, 195-201. PMID: 15665856

9. Yu, X. et al. (2020) Journal of hematology & oncology 13, 40. PMID: 32357935

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CHEK1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: PC-12 cell lysate

References

Datasheet

Anti-Human CHEK1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CHEK1 Polyclonal Antibody (HA503014)

Overview

Images

References

Datasheet

SDS